Fig. 6
From: Development of a novel glycoengineering platform for the rapid production of conjugate vaccines
Mass spectrometry analysis of CmeA glycopeptides A 268AVFDNNNSTLLPGAFATITSEGFIQK293; and B E121DFNR124; purified CmeA was reduced, alkenylated, and treated with sequencing grade trypsin overnight, peptides were then run on LC–MS/MS (Waters). Precursor ion fragmentation shows the loss of HexNAc, Hex, deoxyHex, and deoxyHexNAc which corresponds to one repeating unit of O157. Glycan fragmentation is shown in blue lines and peptide fragmentation is shown in red