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Fig. 6 | Microbial Cell Factories

Fig. 6

From: High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux

Fig. 6

 A. Production and consumption rates of NADPH (estimated from the 13C-MFA results) and specific glycerol uptake rates for each strain. B. FBA results. The y-axis on the left side shows the sum of the fluxes for the main ATP-consuming reactions. The y-axis on the right side shows the percentage of pyruvate entering the mitochondria and the TCA cycle. To do so, the ratio of the fluxes of PYK (the main cytosolic pyruvate producing reaction) and PDH was calculated. Abbreviations: G6PDH, glucose-6-phosphate dehydrogenase; GAME, growth associated maintenance energy; NGAME, non-growth associated maintenance energy; UG/PPP: upper glycolysis and pentose phosphate pathway reactions; Acetyl-CoAcyt: Cytosolic acetyl-CoA production pathway; VPDH/PYK: Ratio between the fluxes of the pyruvate dehydrogenase (PDH) and the pyruvate kinase (PYK) reactions

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Microbial Cell Factories

ISSN: 1475-2859

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