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Fig. 5 | Microbial Cell Factories

Fig. 5

From: High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux

Fig. 5

Flux map for the reference (X-33), PpHP8, and PpHP18 strains growing in glycerol batch cultures at pH 5. The average and the standard deviation of the absolute fluxes for each triplicate experiment are displayed. Fluxes are given in mmol gCDW− 1 h− 1. Abbreviations: G6P: Glucose-6-phosphate; F6P: Fructose-6-phosphate; FBP: Fructose bisphosphate; Glyc: Glycerol; DHAP: Dihydroxyacetone phosphate; G3P: Glyceraldehyde-3-phosphate; R5P: Ribose-5-phosphate; S7P: Sedoheptulose-7-phosphate; E4P: Erythrose-4-phosphate; ABT: D-arabitol; E2: glycolaldehyde moiety of the non-oxidative PPP reactions; E3: dihydroxyacetone moiety of the non-oxidative PPP reactions; 13DPG: 1,3-Bisphosphoglycerate; 3PG: 3-phosphoglycerate; 2PG: 2-phosphoglycerate; PEP: Phosphoenolpyruvate; Pyr: Pyruvate; Acald: Acetaldehyde: Ac: Acetate; AcCoA: Acetyl-CoA; MalCoA: Malonyl-CoA; 3-HP: 3-Hydroxypropionic acid: CIT: Citrate; ICIT: Isocitrate; αKG: α-ketoglutarate; SUCC: Succinate; FUM: Fumarate; MAL: Malate; OAA: Oxaloacetate; GLX: Glyoxylate

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Microbial Cell Factories

ISSN: 1475-2859

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