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Fig. 2 | Microbial Cell Factories

Fig. 2

From: High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux

Fig. 2

Metabolic pathway from pyruvate to 3-HP through cytosolic acetyl-CoA and malonyl-CoA in P. pastoris. The reactions catalysed by all the enzymes linked to overexpressed or deleted genes are displayed. Enzyme abbreviations: Pdc1: Pyruvate decarboxylase 1; Ald: Endogenous cytosolic aldehyde dehydrogenase; ACSSeL641P: Acetyl-CoA synthase from Salmonella enterica harbouring the point mutation L641P to avoid post-translational inhibition of the enzyme by acetylation; Acc1Yl: Acetyl-CoA carboxylase from Y. lipolytica; MCR-CCa: C-terminal domain of the malonyl-CoA reductase from C. aurantiacus; MCR-NCa: N-terminal domain of the malonyl-CoA reductase from C. aurantiacus; cPos5Sc: NADH kinase from S. cerevisiae located on the cytosol; ArDH: Arabitol dehydrogenase. Green and red arrows and enzyme abbreviations indicate whether the corresponding genes were overexpressed or deleted, respectively

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Microbial Cell Factories

ISSN: 1475-2859

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