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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Improving therapeutic protein secretion in the probiotic yeast Saccharomyces boulardii using a multifactorial engineering approach

Fig. 6

Effect of combinatorial and proteomics-driven genome engineering on peptide secretion in Sb. Combinatorial protease knockouts were constructed and the resulting strains were used to express NPA with αMF secretion signal from a high-copy (2µ) yeast origin. Each strain was cultured in triplicate in FlowerPlates in a Biolector II for 48 h at 37 °C. Bars represent the average NPA concentration in three cultivations, and dots represent the NPA concentration in each cultivation

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Microbial Cell Factories

ISSN: 1475-2859

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