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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Improving therapeutic protein secretion in the probiotic yeast Saccharomyces boulardii using a multifactorial engineering approach

Fig. 5

Effect of secretory pathway knockouts on peptide secretion in Sb. Gene candidates involved in yeast’s secretory pathway were deleted from the Sb genome, and the resulting strains were used to express NPA with αMF secretion signal on a high-copy (2µ) yeast origin. Each strain was cultured in triplicate in FlowerPlates in a Biolector II for 48 h at 37 °C. Bars represent the average NPA concentration across three cultivations, and dots represent the NPA concentration in each cultivation

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Microbial Cell Factories

ISSN: 1475-2859

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