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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Improving therapeutic protein secretion in the probiotic yeast Saccharomyces boulardii using a multifactorial engineering approach

Fig. 4

Effect of secretion signal on peptide secretion in Sb. NPA secretion cassettes with 3 native and 3 synthetic secretion signals were cloned into a backbone with a high-copy (2µ) origin. Each strain was cultured in triplicate in FlowerPlates in a Biolector II for 36 h at 37 °C. Bars represent the average NPA concentration across three cultivations, and dots represent the NPA concentration in each cultivation

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Microbial Cell Factories

ISSN: 1475-2859

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