Fig. 3From: Improving therapeutic protein secretion in the probiotic yeast Saccharomyces boulardii using a multifactorial engineering approachEffect of gene copy number on peptide secretion in Sb. NPA with αMF secretion signal was placed on the genome or on plasmids with low-copy (CEN6/ARS4) or high-copy (2µ) yeast origins, and NPA secretion was measured after 48 h. Bars represent the average NPA concentration across three cultivations, and dots represent the NPA concentration in each cultivation for a given strainBack to article page