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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Improving therapeutic protein secretion in the probiotic yeast Saccharomyces boulardii using a multifactorial engineering approach

Fig. 2

NPA secretion in Sb. a SDS-PAGE image of precipitated supernatants of NPA secretor (Sb-NPA) and non-expressing (Sb-Spacer) strains. Each strain was cultured in triplicates in culture tubes at 37 °C for 24 h. The black arrow indicates the band corresponding to tagged NPA. b Secreted NPA titers by Sb-NPA over 48 h in a BioLector microfermenter. Dots represent the NPA concentration for each replicate cultivation and lines connect the average NPA concentration for each timepoint

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