Fig. 6

Effects of three branched-chain amino acids on the riboflavin production of WT and MT strains. A Growth and mycelial color of WT and MT strains in minimal agar medium with three branched-chain amino acids. WT and MT strains were cultivated in minimal agar medium with three branched-chain amino acids (1 mM each) for 2 weeks. B Time course of the riboflavin production in WT and MT strains in minimal liquid medium with three branched-chain amino acids at 1 mM each. WT and MT strains were cultivated in the minimal liquid medium with 1 mM valine for 5 days, and the production of riboflavin was measured. The error bars represent the standard deviation (n = 3). C Specific riboflavin production of the MT strain in minimal liquid medium with three branched-chain amino acids at 1 mM each. MT strain was cultivated for 9 days. Riboflavin concentration and dry mycelial weight were measured. The error bars represent the standard deviation (n = 3). *p < 0.05