Fig. 5
Supplementation of vitamins, micro elements and trace elements to microaerobic A. vinelandii ATCC9046 cultivations. Cultivations were conducted in modified Burk’s medium with 3 g/L yeast extract Roth Batch 1. Standard deviations are given for n ≥ 3 and are in some cases so small, that there are not well recognizable. Significant differences to the reference (3 g/L yeast extract) are marked with asterisks (*** for p < 0.001). If marked with ‘()’, no significance test could be conducted due to n = 1. A, B: For clarity, only every fifth measuring point of the OTR curves is shown. If n = 2, the mean value is shown and the shadow depicts the difference between the two replicates. The horizontal dashed line depicts the OTR value of 2 mmol/L/h for an easier comparison of the nominal cultivation time. C–E: All values are given as mean values, calculated from the following number of replicates: cell dry weight, n ≥ 8; alginate dry weight, n ≥ 6; broth viscosity, n ≥ 6. The flow curves, the mean molecular weight and the acetylation degree are provided as Additional file 1: Fig. S5. Cultivation conditions: 250 mL shake flasks, initial pH = 7.2, filling volume VL = 50 mL, temperature T = 29 °C, shaking frequency n = 165 rpm, shaking diameter d0 = 50 mm