Microbial Cell Factories

Table 2 Analysis of free thiol content, SP cleavage and secondary modifications based on MS

From: Yields and product comparison between Escherichia coli BL21 and W3110 in industrially relevant conditions: anti-c-Met scFv as a case study

Strain	Technique	Location	N° of Cysteine	M_{ox Theor}^a	M_Exp^b	Δ mass	+ 178 Da (5–11%)	−17 Da (12–25%)
BL21	IMAC	Periplasm	4	27478.27	27478.27	0	+ ^c	+
	IMAC	Supernatant		27478.27	27478.27	0	+	+
	CEX	Peak N°2		27478.27	27478.27	0	+	+
	CEX	Peak N°5		27478.27	27478.27	0	+	+
W3110	IMAC	Periplasm		27478.27	27478.27	0	−	+
	IMAC	Supernatant		27478.27	27478.27	0	−	+
	CEX	Peak N°2		27478.27	27478.27	0	−	+
	CEX	Peak N°5		27478.27	27478.27	0	−	+

^aCalculated theoretical oxidised molecular weight (M_OxTheor)
^bExperimental molecular weight (M_Exp)
^cThe sign “ + ” indicates the presence of the modification (+ 178 Da or −17 Da) while “−” indicates the absence of it

Back to article page

ISSN: 1475-2859

Contact us

Submission enquiries: journalsubmissions@springernature.com