Yields and product comparison between Escherichia coli BL21 and W3110 in industrially relevant conditions: anti-c-Met scFv as a case study

Table 1 Comparative analysis of the scFvM produced by the two strains in the different compartments

Strain	Location	Protein concentration (g/L)	Protein purified (mg)	Purification yield (%)	Purification factor^a	Purity (%)
BL21	Periplasm	2.1	0.5	51.8	7.1	92.2
BL21	Supernatant	0.5	45.9	81.4	3.7	91.5
W3110	Periplasm	0.9	0.3	77.5	9.4	91.3
W3110	Supernatant	0.2	26.3	99.6	7.2	92.3

^aThe purification factor was calculated dividing the purification yield (%) over the relative abundance (%) of the target protein in the examined compartment

ISSN: 1475-2859