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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Inducer-free recombinant protein production in Trichoderma reesei: secretory production of endogenous enzymes and heterologous nanobodies using glucose as the sole carbon source

Fig. 2

Endogenous secreted protease and glucoamylase production from T. reesei using an inducer-free system. T. reesei E1AB1 strain was cultivated in shake flasks on an inducing medium containing 3% cellulose and a non-inducing medium containing 3% glucose as a carbon source. E1AB1-XA3, E1AB1-XA3Δcbh1::pep1 (tre74156, aspartic protease) and E1AB1-XA3Δcbh1::gla1 (tre1885, glucoamylase) strains were cultivated on a non-inducing medium containing 3% glucose. a Total secreted proteins after 2, 3, and 4 days of cultivation. b SDS-PAGE analysis of the secreted proteins, the supernatant after 3 days of cultivation were diluted 8-fold and loaded with 5 µL. Open arrowhead indicates the deleted protein, corresponding to the CBH1. Closed arrowheads indicate clearly overexpressed proteins, corresponding to protease and glucoamylase. Error bars indicate standard deviations

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Microbial Cell Factories

ISSN: 1475-2859

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