Table 2 Common promoters used in T. reesei [78, 125]
From: Current genetic strategies to investigate gene functions in Trichoderma reesei
Promoter | Gene function | Remarks |
|---|---|---|
Constitutive | ||
cDNA1 | Unknown | Strong, commonly used |
pdc1 | Pyruvate decarboxylase | Highly expressed on glucose-containing media |
eno1 | Enolase | Highly expressed on glucose-containing media |
tef1 | Transcription elongation factor 1α | Medium strong, activity is lower than cDNA1 promoter |
pki1 | Pyruvate kinase | Medium strength |
pgk1 | 3-phosphoglycerate kinase | Medium strength |
gpd1 | Glyceraldehyde-3-phosphate dehydrogenase | Stable activity on D-glucose |
Tunable | Â | Â |
cbh1 | Cellobiohydrolase (Cel7A) | Inducible with cellulose, sophorose, and lactose. Repressible with D-glucose |
cbh2 | Cellobiohydrolase (Cel6A) | |
egl1 | Endoglucanase (Cel7B) | |
egl2 | Endoglucanase (Cel5A) | |
xyn1 | Xylanase I | Inducible with xylan and D-xylose (concentration dependent). Repressible with the D-glucose and D-xylose (concentration dependent) |
xyn2 | Xylanase II | Inducible with xylan, D-xylose (concentration dependent), xylobiose, cellobiose, and sophorose. Repressible with D-xylose (concentration dependent) and partly with D-glucose |
xyn3 | Xylanase III | Inducible with cellulose, L-sorbose and sophorose. Repressible with D-glucose and D-xylose (concentration dependent) |
tcu1 | Copper transporter | Repressible with copper |
tauD3 | TauD like dioxygenase | Repressible with L-methionine |