Preparation of a Klebsiella pneumoniae conjugate nanovaccine using glycol-engineered Escherichia coli

Table 1 E. coli strains and plasmids used in this study

Strains and plasmids	Genotype descriptions	References
Strains
W3110 WT	W3110 wild-type strain	[48]
W3110∆waaL	waaL gene was knocked out in the W3110 strain	This work
WdlO-d01	wbbH–L gene cluster was knocked out in W3110∆waaL	This work
WdlO-d02	ECA gene cluster was knocked out in WdlO-d01	This work
WdlO-d03	CPS gene cluster was knocked out in WdlO-d01	This work
WdlO-d04	CPS gene cluster was knocked out in WdlO-d02	This work
WdlO-tPS	W3110 ∆waaL∆wbbH–L:: lacI-pglL-SC4573-rrnB	This work
WdlO-tPS01	yfdGHI gene cluster was knocked out in WdlO-tPS	This work
WdlO-tPS02	lpxM gene was knocked out in WdlO-tPS01	This work
Plasmids
pET28a-SpyCatCher4573	Cloning SpyCatCher4573 gene in pET28a	[40]
pACYC184-355-OPS	Cloning 355-OPS gene cluster in pACYC184	[41]
pACYC184-KPO1	Cloning wbbY–Z gene cluster in pACYC184-355-OPS	This work
pCas	The CRISPR system plasmid	[39]
pTargetF	The CRISPR system plasmid	[39]

ISSN: 1475-2859