Fig. 6
From: Production of a 135-residue long N-truncated human keratinocyte growth factor 1 in Escherichia coli
Cell signaling analysis of purified KGF-1135 and KGF-1140 related to the proliferation of MCF-7 cells. (a) Immunoblot analysis of proliferation related cell signaling. Quantitative evaluation of (b) phosphorylated AKT normalized by AKT, (c) phosphorylated ERK normalized by EKR1/2, and (d) phosphorylated Histone H3 normalized by actin. MCF7 cells were serum-starved for 24 h and then treated with KGF-1 for indicated time. Whole cell lysates of each sample were subjected to immunoblotting with the indicated antibodies. * indicated P < 0.05, ** indicated P < 0.01, and *** indicated P < 0.001