Advances in the optimization of central carbon metabolism in metabolic engineering

Wu, Zhenke; Liang, Xiqin; Li, Mingkai; Ma, Mengyu; Zheng, Qiusheng; Li, Defang; An, Tianyue; Wang, Guoli

doi:10.1186/s12934-023-02090-6

Microbial Cell Factories

Table 2 Optimization of CCM in prokaryotic chassis

From: Advances in the optimization of central carbon metabolism in metabolic engineering

Host	Manipulation	CCM involved	Products	References
E. coli	Deletion of PTS system and TyrR repressor	Glycolysis	L-Tyrosine	[52]
E. coli	Deletion of PTS system and TyrR repressor	Glycolysis	Melanin	[53]
E. coli	Deletion of PTS system and TyrR repressor	Glycolysis	L-DOPA	[54]
E. coli	Deletion of PTS system and repression of the activity of Alanine: H⁺ symporter	Glycolysis	β-alanine	[51]
E. coli	Deletion of ZWF and TPIA	Glycolysis	3-Hydroxypropionic acid	[62]
E. coli	Construction of variants of the pyruvate dehydrogenase complex and deletion of LDHA and POXB	Glycolysis	Pyruvate	[68]
E. coli	Regulation of carbon distribution by a thermal switch system	Glycolysis	L-Threonine	[70]
E. coli	Design and construction of non-oxidative glycolysis pathway	Glycolysis	Acetyl-CoA	[69]
E. coli	Silence of a dozen or more CCM enzymes by CRISPR system	Glycolysis and TCA cycle	(2 S)-Naringenin	[63]
E. coli	High-throughput screening of CCM key enzymes and fine-tuning of coding genes by CRISPR silencing system	Glycolysis and TCA cycle	(2 S)-Pinocembrin	[64]
E. coli	High-throughput screening of CCM key enzymes and fine-tuning of coding genes by CRISPR silencing system	Glycolysis and TCA cycle	Medium chain fatty acids	[65]
E. coli	Expression of response regulator DR1558 from D. radiodurans	Glycolysis and TCA cycle	Poly‑3‑hydroxybutyrate	[24]
E. coli	Introduction of metabolic toggle switch	Glycolysis and TCA cycle	3-Hydroxypropionic acid	[71]
E. coli	Replacement of PTS system with galactose translocation system	Glycolysis and TCA cycle	Fumaric acid	[56]
E. coli	Substitution of PTS system with independent glucose transport system and overexpression of PK	Glycolysis and PPP	L-tryptophan	[57]
E. coli	ZWF deletion	Glycolysis and PPP	Lycopene	[59]
E. coli	ZWF deletion	Glycolysis and PPP	β-Carotene	[61]
E. coli	Overexpression of TPIA and FBAA	TCA cycle	Poly‑3‑hydroxybutyrate	[66]
E. coli	Introduction of the efficient citrate synthase variant	TCA cycle	Acetate	[67]
E. coli	Deletion of PTS system, PYK, PEPC and Malic enzyme	TCA cycle	Succinate	[55]
E. coli	Deletion of PGI and overexpression of ACS	PPP	Riboflavin	[60]
C. glutamicum	PEPC deletion	Glycolysis	(3R)-Acetoin	[73]
C. glutamicum	PEPC deletion	Glycolysis	Isopropanol	[74]
C. glutamicum	Deletion of PTS system and depression of myo-inositol catabolism repressor IolR	Glycolysis	L-Serine	[75]
C. glutamicum	Introduction of myo-inositol/proton symporter variant and downregulate of Cs	Glycolysis	Hydroxybenzoic acids	[76]
C. glutamicum	Reduction of CS catalysis activity	TCA cycle	Naringenin	[72]
B. licheniformis	Deletion of PTS system and PYK	Glycolysis	2-phenylethanol	[78]
B. licheniformis	Overexpression of PDH and Cs and deletion of pyruvate formate-lyase gene	TCA cycle	Poly-γ-glutamic acid	[77]
Z. mobilis	Ectopic expression of PDC to construct a CCM control-valve	Glycolysis	Lactate and isobutanol	[83]
M. succiniciproducens	Deletion of fructose PTS system	Glycolysis	Succinic acid	[84]
G. thermoglucosidasius	Deletion of transcriptional regulator ccpN_Gtg	Glycolysis and PPP	Riboflavin	[85]
B. subtilis	Establishment of a pyruvate-responsive genetic circuit	Glycolysis and TCA cycle	Glucaric acid	[81]
P. putida	Introduction of genes for rhamnolipid synthesis	Glycolysis and TCA cycle	Rhamnolipid	[79]
S. pogona	Overexpression of the transcriptional regulator Sp13016	Glycolysis, PPP and TCA cycle	Butenyl-spinosyn	[86]
A. pretiosum	ZWF deletion	PPP	Ansamitocins	[82]
M. extorquens	Construction of a sensor by transcriptional regulator QscR	PPP and TCA cycle	Acetyl-CoA and Mevalonate	[87]

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ISSN: 1475-2859

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