Fig. 5

Biosensor response during β-lactoglobulin and β-casein production. a, β-lactoglobulin and b, β-casein under methanol induction at 72, 90, 95, 110, 115 and 130 h. Fluorescence is indicated as relative fluorescence units (RFU) β-lactoglobulin and β-casein abundance by protein concentration and proteomics analysis in the supernatant, respectively. Correlation between secreted protein abundance and intracellular GFP fluorescence shown in framed graphs. Data are presented as mean and standard deviation of biological replicates and analysed using one-way ANOVA with multiple comparisons. Dash line indicates background fluorescence at 485–520 nm. *Significantly different to all colonies at 130 h (p < 0.01). ^# Significantly different to colonies 15, 19, 23, 24, 26, 29, 31, 32 and protease deficient strain (DP) at 130 h (p < 0.01). ^Δ Significantly different to all colonies except 1, 2, 4, 8 and 9 at 130 h (p < 0.01)