A novel thermostable TP-84 capsule depolymerase: a method for rapid polyethyleneimine processing of a bacteriophage-expressed proteins

Table 1 Bacteriophage TP-84_26 potential gene variants and encoded depolymerases properties

Depolymerase variant	CDS length (bp) (including STOP)	Location in the genome (bp)^a	Polypeptide length (aa)	Predicted polypeptide MW (kDa)	Experimentally determined polypeptide MW (kDa)	Quaternary structure^c	Predicted isoelectric point
Depolymerase (+ 8 aa)	3000	20155–23154	999	113.1	ND	Tetramer	5.47
Depolymerase full length	2976	20179–23154	991	112.2	115 ± 5^b	Tetramer	5.49
Depolymerase (-6 aa)	2958	20197–23154	985	111.4	115 ± 5^b	Tetramer	5.23
Depolymerase (-7 aa)	2955	20200–23154	984	111.3	115 ± 5^b	Tetramer	5.23
Depolymerase (-11 aa)	2943	20212–23154	980	110.8	ND	Tetramer	5.23
Depolymerase (-13 aa)	2937	20218–23154	978	110.5	ND	Tetramer	5.16
Depolymerase (-29 aa)	2889	20 266–23154	962	108.5	110 ± 5	Tetramer	5.14

^aas based on several LC–MS analysis, it is conceived that the depolymerase C-terminal sequence is not clipped off for any of the potential variants
^bresolution of those variants on polyacrylamide gels is within error range of SDS-PAGE
^cthis experiment was conducted using a mixture of depolymerase variants, as obtained from purification procedure

ISSN: 1475-2859