Fig. 6

A Center of spectral mass (CMS) of the Trp emission (Appendix B) in the absence (control) or in the presence of N-L. These data were applied to calculate the ΔCMS = CMS_+N-L – CMS_control and all the ΔCMS values were statistically different from zero (p < 0.05). B Speculative model of the mechanism of action of N-L, whose presence could place the his-tag in a more convenient way for its binding to the Ni²⁺ in the column. GWH1-GFP-H6 is shown at the top and GWH1-INFγ-H6 at the bottom. Colour codes are as it follows: yellow (antimicrobial peptide), red (his-tag) purple (scaffold protein) and green (N-L molecules)