Table 3 Strains and plasmids used in this study
Strain/Plasmid | Genotype/Description | Source |
|---|---|---|
E. coli DH5α | F– φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK–, mK+) phoA supE44 λ–thi-1 gyrA96 relA1 | Thermo Fisher Scientific (USA) |
E. coli BL21 (DE3) | F–ompT gal dcm lon hsdSB(rB–mB–) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K−12(λS) | Thermo Fisher Scientific (USA) |
E. coli Tuner (DE3) | F–ompT hsdSB (rB– mB–) gal dcm lacY1(DE3) | Novagen®, Merck (Germany) |
E. coli ArcticExpress | F–ompT hsdS (rB–mB–) dcm+ Tetrgal λ(DE3) endA Hte [cpn10 cpn60 Gentr] | Agilent Technologies (USA) |
Vibrio natriegens Vmax™ | Vibrio natriegens 14,048 dns::LacI-T7-RNAP | Telesis Bio (USA) |
pET28a MsAA NTag/CTag/noTag | Derived from pET28a-eforRED[37], with coding sequence of MsAA with N- or C-terminal Strep-Tag, or without affinity tag | This study |
pGKJE8 | Arabinose-induced expression of dnaK/J/grpE; tetracycline-induced expression of groEL/S | Takara Bio Europe (France) |
pKJE7 | Arabinose-induced expression of dnaK/J/grpE | |
pGTf2 | Tetracycline-induced expression of dnaK/J/grpE and tig (Trigger factor) | |
pTf16 | Arabinose-induced expression of tig | |
pGro7 | Arabinose-induced expression of groEL/S |