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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Chaperone assisted recombinant expression of a mycobacterial aminoacylase in Vibrio natriegens and Escherichia coli capable of N-lauroyl-L-amino acid synthesis

Fig. 3

SDS-PAGE analysis of cell extracts obtained from V. natriegens Vmaxâ„¢ after expression of MsAA variants

Cells were grown in BHIv2 medium for 5 h at 30 °C. The arrow indicates the position of MsAA variants

Lane 1: protein marker (BlueEasy Prestained Protein Marker, Nippon Genetics); lane 2: cell-free extract with MsAA NTag; lane 3: insoluble fraction with MsAA NTag; lane 4: cell-free extract with MsAA CTag; lane 5: insoluble fraction with MsAA CTag; lane 6: cell-free extract with MsAA noTag; lane 7: insoluble fraction with MsAA noTag; lane 8: cell-free extract of empty vector control; lane 9: insoluble fraction of empty vector control

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Microbial Cell Factories

ISSN: 1475-2859

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