Fig. 8
From: Discovery of novel amino acid production traits by evolution of synthetic co-cultures
Characterization of l-arginine production in monocultures of A ∆LEU ARG++ and B ∆LEU ARG++ ∆argTUV in CGXII with 2% (w/v) d-glucose and 3 mM l-leucine. After precultivation in CGXII defined medium with 111 mM d-glucose and 3 mM l-leucine, the cell suspension of one culture was used to inoculate eight wells of a BioLector Flowerplate. Upon sampling, a full well was harvested for each replicate at a certain time point. l-arginine and l-leucine were quantified in cell-free supernatants via HPLC and mean values are represented including the corresponding standard deviation. Mean values and standard deviations of biological triplicates are shown as lines and shaded areas, respectively