Table 1 Strains and plasmids used in this study
Name | Relevant strain and genotype | Sources |
|---|---|---|
Strains | ||
B. subtilis 168 | trpC2 | Donated by Tianjin University [22] |
BS168N | trpC2, ΔaraR::Para-neo | Donated by Tianjin University [22] |
BS168NUm | trpC2, ΔaraR::Para-neo, Δupp::cat-araR | Donated by Tianjin University [22] |
E. coli DH5α | F–, Φ80lacZΔM15, Δ(lacZYA-argF), U16, recA1, endA1, hsdR17 (rK–, mK+), phoA, supE44, λ–, thi1, gyrA96, relA1 | Laboratory stock |
BSH1 | BS168N, ΔhemX | This study |
BSH1Ym | BS168N, ΔhemX, ΔywfM::D-cat-araR | This study |
BSH2 | BS168N, ΔhemX, ΔywfM | This study |
BSH3 | BS168N, ΔhemX, ΔywfM::P43-hemA | This study |
BSH4 | BS168N, ΔhemX, ΔywfM::P43-hemA, ΔrocG | This study |
BSH51 | BS168N, ΔhemX, ΔywfM::P43-hemA, ΔrocG::P43-ALASRp | This study |
BSH52 | BS168N, ΔhemX, ΔywfM::P43-hemA, ΔrocG::P43-ALASRc | This study |
BSH53 | BS168N, ΔhemX, ΔywfM::P43-hemA, ΔrocG::P43-ALASBj | This study |
BSH54 | BS168N, ΔhemX, ΔywfM::P43-hemA, ΔrocG::P43-ALASAt | This study |
BSH6 | BSH53, ΔgcvTP | This study |
BSH7 | BSH53, ΔgcvTP::PlapS-hemCDB | This study |
BSH8 | BSH7, ΔywjI::PlapS-ccmABCEc | This study |
BSH9 | BSH7, ΔywjI::PlapS-ccmABCEc, ΔnasF | This study |
BSH10 | BSH7, ΔywjI::PlapS-ccmABCEc, ΔnasF, ΔhmoA | This study |
BSH11 | BSH7, ΔywjI::PlapS-ccmABCEc, ΔnasF, ΔhmoA, ΔhmoB | This study |
Plasmids | ||
pUC57-1.8k-1 | AmpR, containing the alaS gene from R. palustris, synthesized by Genecreate Biotech | This work |
pUC57-1.8k-2 | AmpR, containing the alaS gene from R. capsulatus, synthesized by Genecreate Biotech | This work |
pUC57-1.8k-3 | AmpR, containing the alaS gene from B. japonicum, synthesized by Genecreate Biotech | This work |
pUC57-1.8k-4 | AmpR, containing the alaS gene from A. tumefaciens, synthesized by Genecreate Biotech | This work |
pUC57-1.8k-P1 | AmpR, containing promoter PlapS | Donated by Tianjin University [22] |