Fig. 2

The expression of small laccases genes in S. lividans host organism. (a) Experimental set up for the small laccases production and secretion studies. Bacteria were cultivated with the growth medium containing 0 or 100 µM of CuSO₄. Samples at defined time points were harvested and the samples without CuSO₄ were divided into two aliquots. The first aliquot was saved for control measurements. The second aliquot was reconstituted by supplementing with 100 µM CuSO₄, incubated overnight and measured on the next day. Reconstituted samples were named medium (R) and cells (R). (b) Secretion of AmLac, (c) secretion of ScLac and (d) secretion of SvLac. The spores were cultivated in the growth medium containing 100 µM of CuSO₄. The samples were taken at defined time points, medium and cells were separated by centrifugation and activity was measured spectrophotometrically. The wet cell weight (WCW) was measured to follow bacterial growth. After 24 h no cell growth was observed. The activity measurements are an average of three biological replicates in three technical replicates each