Fig. 4
a Growth profiles of 90 isolated evolved strains (EVS) cultivated in 0.2 mL synthetic medium containing 15% v/v pure glycerol. The average growth curve of the EVS (WTev.pure, n = 58; EMSev.pure, n = 12; WTev.crude, n = 20) is depicted in red while the green curve stands for the parental strain (WTpar). Standard deviation is indicated with error bars for the WTpar whereas for the 90 EVS with pink shading. OD600nm was monitored using a microtiter plate reader. Number of replicates (n = 3). b Lipid fluorescence and distribution per cell after 48 h of cultivation, using flow cytometry and bodipy dye (493/503 nm). Minimum and maximum fluorescence distribution curves of 90 EVS are shown in two red curves, WTpar in green, while unstained WTpar cells were used as control (blue)