Fig. 7
From: Engineering Escherichia coli for efficient assembly of heme proteins
Application of Ec-M13 strain for whole-cell P450 bioconversion for nonanedioic acid production. A The synthetic pathway of nonanedioic acid from nonanoic acid. B Nonanedioic acid production. Three CYP153A subfamily cytochrome P450 (CYP) enzymes derived from M. marinum (MmCYP), Polaromonas sp. JS666 (PsCYP), and M. aquaeolei (MaqCYP) were tested. P. putida putidaredoxin reductase and putidaredoxin (PpCamAB) and P. megaterium NADPH-cytochrome P450 oxidoreductase (PmNCP) are NADH- and NADPH-dependent electron transport proteins, respectively, for reconstituting the activity of CYP enzymes