Fig. 7

GM1-ganglioside binding assay (GM1 ELISA) of LTB-VP1. A ELISA was conducted as described earlier. The plates coated with GM1 monosialoganglioside were incubated with the protein preparation of two selected LTB-VP1 transformants and then cross-reacted with anti-LTB antiserum. B Plates are shown to demonstrate colorimetric changes in the well, in contrast with samples from the 30 °C cultures. PC and NC indicate that the E. coli-expressed LTB as a positive control and a mock transformant as a negative control, respectively. Well number 1 indicates the sample of protein preparation without dilution and well numbers (2—8) indicate samples with twofold dilution of the original protein preparation