Fig. 6From: Improving expression and assembly of difficult-to-express heterologous proteins in Saccharomyces cerevisiae by culturing at a sub-physiological temperatureGM1-ganglioside binding assay (GM1 ELISA) of LTB-EDIII2. The ELISA was conducted by coating 96-wells with GM1 monosialoganglioside as the receptor molecule. Y-axis indicates the percentage of pentameric LTB-EDIII2 in the sample preparations. The coated plates were incubated with the protein preparation of transformant #8 and then cross-reacted with anti-LTB antiserumBack to article page