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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Improving expression and assembly of difficult-to-express heterologous proteins in Saccharomyces cerevisiae by culturing at a sub-physiological temperature

Fig. 6

GM1-ganglioside binding assay (GM1 ELISA) of LTB-EDIII2. The ELISA was conducted by coating 96-wells with GM1 monosialoganglioside as the receptor molecule. Y-axis indicates the percentage of pentameric LTB-EDIII2 in the sample preparations. The coated plates were incubated with the protein preparation of transformant #8 and then cross-reacted with anti-LTB antiserum

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