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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Improving expression and assembly of difficult-to-express heterologous proteins in Saccharomyces cerevisiae by culturing at a sub-physiological temperature

Fig. 5

Western blot analysis of temporal expression of LTB-EDIII2 from transformant #8. A LTB-EDIII2 expression was resolved under non-denaturing condition at 20 °C and 30 °C. B SDS-PAGE gel showed that an equal amount of protein was loaded on each lane. Lane 1: purified E. coli-expressed LTB as a positive control; Lane 2: a mock transformant cultured for 3 days as a negative control. Proteins were prepared from transformant #8, and cultured for 1, 3, 5 days after inoculation to the expression medium at 20 °C (lanes 3, 5, and 7, respectively) and 30 °C (lanes 4, 6, and 8, respectively)

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Microbial Cell Factories

ISSN: 1475-2859

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