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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Improving expression and assembly of difficult-to-express heterologous proteins in Saccharomyces cerevisiae by culturing at a sub-physiological temperature

Fig. 4

Western blot analysis of three selected LTB-EDIII2 transformants. A LTB-EDIII2 detection under denatured condition. B LTB-EDIII2 detection under non-denatured condition. Densitometry analysis of cross-reacting LTB-EDIII2 bands in the corresponding blot is shown on the right panel. C SDS-PAGE gel shows that an equal amount of protein was loaded on each lane. Lane 1: purified E. coli-expressed EDIII2 as a positive control; Lane 2: a mock transformant as a negative control; Lanes 3, 5 and 7: LTB-EDIII2 transformants #1, #8, and #9 cultured at 20 °C; Lanes 4, 6 and 8: LTB-EDIII2 transformants #1, #8, and #9 cultured at 30 °C. Proteins were prepared from cells cultured for 3 days after the inoculation to the expression medium

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Microbial Cell Factories

ISSN: 1475-2859

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