Fig. 3

Northern blot analysis (A) and Quantitative real time RT-PCR (qRT-PCR) analysis (B) of LTB-EDIII₂ in a selected transformant (#8) under 20 °C and 30 °C conditions. A 20 µg total RNA was loaded on each lane. RNA preparations from cells harvested at day 1, 3, and 5 days after cultivation at 20 °C (Lanes 1–3, respectively) and 30 °C (Lanes 4–6, respectively). GPD was used as an internal control and rRNAs are shown to indicate equal amount of RNA loaded on each lane. B qRT-PCR results of changes in expression of LTB-EDIII₂ under 20 °C and 30 °C conditions are shown. Error bars indicate standard deviation based on three independent measurements. ** indicates statistically significant difference between two groups, according to t-test at p = 0.01