Fig. 2

A Northern blot analysis of 12 transformants. 30 µg of total RNA was loaded on each lane. LTB-EDIII₂ was used as the probe to detect LTB-EDIII₂ expression. Lanes 1–12: LTB-EDIII₂ transformants were randomly selected and numbered from 1 to 12. B Temporal expression of LTB-EDIII₂ transcript in three selected transformants (transformants #1, #8, and #9 in panel A). Lane numbers indicate the number of days after cultivation at 30 °C and strains are indicated at the top of the lanes. Glyceraldehyde-3-phosphate dehydrogenase gene (GPD) was used as an internal control and bands representing rRNAs in ethidium bromide strained-gel (rRNA) are shown to indicate an equal amount of RNA loaded on each lane