Fig. 1

Expression analysis of yeast codon optimized eGFP under two different expression conditions using FACS (A) and Western blot analysis (B and C). A FACS analysis of expressed eGFP. Inset: yeast cells cultured at 20 °C (left) and 30 °C condition (right) show eGFP expression under UV light. B Western blot of yeast expressing eGFP in 20 °C and 30 °C conditions. Lane 1: purified eGFP; lane 2: S. cerevisiae 2805 strain; lanes 3–5: protein preparation from eGFP-expressing yeast cells harvested after 1, 3, and 5 days, respectively, at 20 °C. Lanes 6–8: protein preparation from eGFP-expressing yeast cells harvested after 1, 3, and 5 days, respectively, at 30 °C. C SDS-PAGE twin gel showing that a similar amount of protein was loaded on each lane