Fig. 1
De novo biosynthetic pathway for resveratrol production in engineered S. cerevisiae. Both tyrosine and phenylalanine were converted by bi-functional phenylalanine/tyrosine lyase from R. toruloides (RtPAL/TAL). ARO4K229L: 3-deoxy-D-arabinoheptulosonate-7-phosphate (DAHP) synthase mutant; EcAROL: shikimate kinase from E. coli; ARO2: chorismate synthase; ARO7G141S: chorismate mutase mutant; SeACSL641P: acetyl-CoA synthase mutant from S. enterica; ACC1S659A/S1157A: acetyl-CoA carboxylase mutant; AtC4H&AtCPR1: cinnamate-4-hydroxylase and cytochrome P450 reductase from A. thaliana; Pc4CL: p-coumaroyl coenzyme A ligase from P. crispum; VvSTS: stilbene synthase from V. vinifera. The solid arrow indicated one enzymatic step, the dash arrow indicated multiple enzymatic steps, and the green arrow in bold indicated multi-copy integration of pathway genes. The green letters represent heterogeneous pathway of resveratrol, others represent the genes involved in host engineering, with the red ones from S. cerevisiae were overexpressed, purple ones from other organisms were overexpressed, and grey ones from S. cerevisiae were knocked out