Enhancement of herbicolin A production by integrated fermentation optimization and strain engineering in Pantoea agglomerans ZJU23

Table 4 Variance analysis results of Box-Behnken design for HA production

Source	Sum of squares	Degree of freedom	Mean Square	F-value	P-value
Model	20,727.32	9	2303.04	32.19	0.0007
A-Corn Steep Liquor	6464.19	1	6464.19	90.36	0.0002
B-Initial pH	4729.58	1	4729.58	66.11	0.0005
C-Temperature	1251.49	1	1251.49	17.49	0.0086
AB	778.76	1	778.76	10.89	0.0215
AC	193.17	1	193.17	2.70	0.1613
BC	1607.56	1	1607.56	22.47	0.0051
A²	2302.63	1	2302.63	32.19	0.0024
B²	2557.28	1	2557.28	35.75	0.0019
C²	1708.63	1	1708.63	23.88	0.0045
Residual	357.69	5	71.54
Lack of Fit	356.31	3	118.77	172.27	0.0058
Pure Error	1.38	2	0.6894
Cor Total	21,085.01	14	R²	0.9830
Std. Dev	8.46		Adjusted R²	0.9525
Mean	132.07		Predicted R²	0.7295
C.V. %	6.40		Adeq Precision	15.2738

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