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Table 3 Box-Behnken design for HA production

From: Enhancement of herbicolin A production by integrated fermentation optimization and strain engineering in Pantoea agglomerans ZJU23

Factor

A

Corn Steep Liquor (g/L)

B

Initial pH

C

Temperature (°C)

Response

HA (mg/L)

Std

Run

Pattern

8

1

 + 0 + 

15.0

6.5

26

133.67

9

2

0 − −

11.5

5.0

20

177.72

15

3

0 0 0

11.5

6.5

23

169.99

14

4

0 0 0

11.5

6.5

23

171.62

10

5

0 + −

11.5

8.0

20

99.52

5

6

− 0 −

8.0

6.5

20

101.26

1

7

− − 0

8.0

5.0

23

104.05

3

8

− + 0

8.0

8.0

23

72.81

11

9

0 − + 

11.5

5.0

26

106.53

13

10

0 0 0

11.5

6.5

23

171.10

7

11

− 0 + 

8.0

6.5

26

96.23

2

12

 + − 0

15.0

5.0

23

194.32

12

13

0 +  + 

11.5

8.0

26

108.52

6

14

 + 0 −

15.0

6.5

20

166.50

4

15

 +  + 0

15.0

8.0

23

107.26