Fig. 5
From: Improving the production of the micafungin precursor FR901379 in an industrial production strain
Functional verification of self-resistance genes related to FR901379. A The transcription levels and homologous genes of the genes CEfks1 and CEfks2; MKF: FR901379 high-producing condition; LPM: FR901379 low-producing condition; FPKM: Fragments Per Kilobase of exon model per Million mapped fragments. B Schematic diagram for the construction of CEfks1 and CEfks2 disruption mutants. C FR901379 titers were quantified in the CEfks disruption strains and CEfks2 overexpression strains; WT: C. empetri MEFC09; ∆fks1: MEFC09-∆CEfks1; ∆fks2: MEFC09-∆CEfks2; OEfks2: MEFC09::CEfks2. D Images of mycelial morphology of the CEfks1 and CEfks2 disruption strains; PDA: the strains were grown on PDA medium; SM: stereo microscope images (8 × objective lens) of mycelia of the cultivation broth, all scale bars represent 2 mm; SEM: scanning electron microscopic images of CEfks disruption strains