High-efficiency production of the antimicrobial peptide pediocin PA-1 in metabolically engineered Corynebacterium glutamicum using a microaerobic process at acidic pH and elevated levels of bivalent calcium ions

Table. 2 Impact of recombinant pediocin production in C. glutamicum on global gene expression

Locus tag	Gene	Gene description	log2-fold change
Up regulated
	pedA	Pediocin biosynthesis	12.8
	pedD	Pediocin biosynthesis	12.8
CGL_RS05840		PspC-domain containing protein	3.0
CGL_RS03930	pdxS	Pyridoxal 5'-phosphate synthase lyase	0.8
CGL_RS04960	aroF	3-Deoxy-7-phosphoheptulonate synthase	0.7
Down regulated
CGL_RS09745	bioY	Biotin transporter	1.6
CGL_RS09750		ABC transporter ATP-binding protein	0.6
CGL_RS10490		ABC transporter substrate-binding protein	0.6

C. glutamicum CR099 pXMJ19 P_tac pedACD^Cg was compared with CR099 pXMJ19 expressing the empty plasmid as control. The cultures were sampled after 13 h (Fig. 1). The data comprise genes that found significantly changed in expression (log₂-fold change > 1.0). In addition, weakly affected genes are included (1 > log₂-fold > 0.6). Data significance was verified by a t test (p < 0.1). n = 3

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