High-efficiency production of the antimicrobial peptide pediocin PA-1 in metabolically engineered Corynebacterium glutamicum using a microaerobic process at acidic pH and elevated levels of bivalent calcium ions

Christmann, Jens; Cao, Peng; Becker, Judith; Desiderato, Christian K.; Goldbeck, Oliver; Riedel, Christian U.; Kohlstedt, Michael; Wittmann, Christoph

doi:10.1186/s12934-023-02044-y

Table 1 Strains and plasmids used in this study

From: High-efficiency production of the antimicrobial peptide pediocin PA-1 in metabolically engineered Corynebacterium glutamicum using a microaerobic process at acidic pH and elevated levels of bivalent calcium ions

Strain	Description	Refs.
E. coli
DH10B	Cloning host	[98]
L. innocua
pIMK2	Sensor strain expressing the plasmid pIMK2 kan^R	[23]
pNZ44	Sensor strain expressing the plasmid pNZ44 cm^R	[23]
C. glutamicum
CR099	Genome reduced derivative of strain ATCC 13,032 with deletion of ΔCGP1-3 and ΔISCg1-2	[96]
CR099 pEKEx2 P_tac pedACD^Cg	CR099 with episomal expression of codon-optimized pediocin operon pedACD^Cg from Pediococcus acidilactici under control of P_tac	[23]
CR099 pXMJ19 P_tac pedACD^Cg	CR099 with episomal expression of the codon-optimized pediocin operon from P. acidilactici under control of P_tac	[23]
CR099 pXMJ19	CR099 with episomal expression of empty pXMJ19	[23]
CR099 pClik 5α P_tuf pedACD^Cg	CR099 with episomal expression of the codon-optimized pediocin operon from P. acidilactici under control of P_tuf	This work
CR099 pEKEx2 P_tac pedA^M31LCD^Cg	CR099 with episomal expression of the codon-optimized pediocin operon from P. acidilactici under control of P_tac including mutated pedA^M31L	This work
CR099 pClik 5α	CR099 with episomal expression of empty pClik 5α	This work
CR099 pClik 5α vgb	CR099 with episomal expression of the heterologous gene vgb from Vitreoscilla spp. [52] under control of P_tuf	This work
CR099 pClik 5α dps	CR099 with episomal expression of the native C. glutamicum gene dps [49] under control of P_tuf	This work
CR099 pClik 5α mcbR	CR099 with episomal expression of the native C. glutamicum gene mcbR [49] under control P_tuf	This work
CR099 pClik 5α mpx	CR099 with episomal expression of the native C. glutamicum gene mpx [47] under control of P_tuf	This work
CR099 pClik 5α mshA	CR099 with episomal expression of the native C. glutamicum gene mshA [48] under control of P_tuf	This work
CR099 pClik 5α katA	CR099 with episomal expression of the native C. glutamicum gene katA [49] under control of P_tuf	This work
CR099 pClik 5α zwf	CR099 with episomal expression of the native C. glutamicum gene zwf [99] under control of P_tuf	This work
CR099 pClik 5α zwf ^ATG	CR099 with episomal expression of the native C. glutamicum gene zwf under control of the translational start codon ATG [99] and P_tuf	This work
CR099 pClik 5α ATG zwf ^{ATG, A243T}	CR099 with episomal expression of the mutated variant zwf ^A243T [56] under control of the translational start codon ATG [99] and P_tuf	This work
CR099 pClik 5α P_H30 DR1558	CR099 with episomal expression of the heterologous gene DR1558 from Deinococcus radiodurans [50] under control of the synthetic promotor P_H30	This work
CR099 pClik 5α P_tuf DR1558	CR099 with episomal expression of the heterologous gene DR1558 from Deinococcus radiodurans [50] under control of P_tuf	This work
Plasmids
pClik5α MCS	Episomal vector, ORI^Cg, ORI^Ec, kan^R	[97]
pXMJ19	Episomal vector, P_tacI lacI, ORI^Cg, ORI^Ec, cm^R	[23]
pXMJ19 P_tac pedACD^Cg	Episomal expression of pedACD^Cg under control of P_tac	[23]
pClik 5α P_tuf pedACD^Cg	Episomal expression of pedACD^Cg under control of P_tuf	This work
pClik 5α P_tuf vgb	Episomal expression of vgb [52] under control of P_tuf	This work
pClik 5α P_tuf dps	Episomal expression of dps [49] under control of P_tuf	This work
pClik 5α P_tuf mcbR	Episomal expression of mcbR [49] under control of P_tuf	This work
pClik 5α P_tuf mpx	Episomal expression of mpx [47] under control of P_tuf	This work
pClik 5α P_tuf mshA	Episomal expression of mshA [48] under control of P_tuf	This work
pClik 5α P_tuf katA	Episomal expression of katA [49] under control of P_tuf	This work
pClik 5α P_tuf zwf	Episomal expression of zwf under control of P_tuf	This work
pClik 5α P_tuf zwf ^ATG	Episomal expression of zwf under control of the start codon ATG [12] and P_tuf	This work
pClik 5α P_tuf zwf ^{ATG, A243T}	Episomal expression of the mutated variant zwf ^A243T [56] under control of the start codon ATG [12] and P_tuf	This work
pClik 5α P_H30 DR1558	Episomal expression of DR1558 [50] under control of P_H30	This work
pClik 5α P_tuf DR1558	Episomal expression of DR1558 [50] under control of P_tuf	This work

Back to article page

ISSN: 1475-2859

Contact us

Submission enquiries: journalsubmissions@springernature.com

Microbial Cell Factories

Contact us