Fig. 5

Optimized pediocin PA-1 production in lab scale bioreactors in batch mode using recombinant C. glutamicum. As carbon source, the medium contained 80 g L⁻¹ of glucose and 15 g L⁻¹ of yeast extract. After an initial growth phase (10% DO, pH 6.5), the process was shifted to pediocin PA-1 production (2.5% DO, pH 5.7). The data comprise C. glutamicum CR099 pXMJ19 P_tac pedACD^Cg which expressed the cluster under control of the P_tac promotor and was induced by 0.2 mM IPTG at process start (A, B). In addition, C. glutamicum CR099 pClik 5α P_tuf pedACD^Cg was used, expressing the cluster under control of the constitutive P_tuf promotor, so that no inducer was required (C, D). n = 2