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Fig. 4 | Microbial Cell Factories

Fig. 4

From: High-efficiency production of the antimicrobial peptide pediocin PA-1 in metabolically engineered Corynebacterium glutamicum using a microaerobic process at acidic pH and elevated levels of bivalent calcium ions

Fig. 4

Evaluation of ten gene candidates to support growth of the chassis strain C. glutamicum CR099 at pH 5.9 and low oxygen supply. The cultures were conducted in a miniaturized microtiter plate system with online sensing of cell concentration. The different gene candidates were previously studied to increase the tolerance of C. glutamicum and encoded for homologous and heterologous proteins: dps, iron-storage protein Dps; mpx, mycothiol peroxidase; katA, catalase; mcbR, transcriptional repressor McbR; mshA, D-inositol 3-phosphate glycosyltransferase [47,48,49]; DR1558, transcriptional response regulator [50] (Table 2). Each gene was episomally expressed under control of the constitutive promoters Ptuf and PH30. A strain expressing the empty vector served as control. n = 3

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Microbial Cell Factories

ISSN: 1475-2859

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