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Fig. 3 | Microbial Cell Factories

Fig. 3

From: High-efficiency production of the antimicrobial peptide pediocin PA-1 in metabolically engineered Corynebacterium glutamicum using a microaerobic process at acidic pH and elevated levels of bivalent calcium ions

Fig. 3

Improvement of pediocin production in recombinant C. glutamicum CR099 pXMJ19 Ptac pedACDCg by medium optimization. The cultures were conducted in a miniaturized microtiter plate system with online sensing of cell concentration, pH value, and DO level (A). In a first series of experiments, the pH was adjusted to different values between 5.7 and 6.5 using 200 mM MES (A). Impact of the pH value on pediocin production (B). The data display the final pediocin activity in culture supernatant. In addition, the specific production efficiency is given, normalized to the period of oxygen limitation (required for pediocin accumulation) and the average cell concentration during this phase. In a second series of experiments, the impact of CaCl2 on pediocin production was assessed (C). Different levels of were supplemented to the medium. The pH was kept at 5.9. The data display the final pediocin activity in culture supernatant. n = 3

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Microbial Cell Factories

ISSN: 1475-2859

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