Fig. 1

Pediocin production in shake flasks using recombinant C. glutamicum CR099 pXMJ19 P_tac pedACD^Cg. The data show growth and production on TY medium, containing 20 g L⁻¹ of yeast extract, 16 g L⁻¹ of bacto-tryptone, additionally supplemented with 20 g L⁻¹ of glucose and 0.2 mM of IPTG after 2 h, indicated by a vertical dashed line A. The non-producing reference strain C. glutamicum CR099 pXMJ19, expressing the empty vector, is shown for comparison (B). In addition, GY medium was used to produce pediocin (C). The medium contained 10 g L⁻¹ of glucose, 10 g L⁻¹ of yeast extract, mineral salts, and vitamins. It was supplemented with IPTG (0.2 mM), two hours after the start. In addition, the corresponding total carbon content of the TY (D) and the GY medium (E) is given. For the complex ingredients, the total carbon content was estimated from the composition given by the supplier, whereby carbohydrates were considered as glucose units [34]. n = 3