Fig. 1

Molecular confirmation of recombinant construct and the protein expression in E. coli. a) Recombinant HBc VLP-E and recombinant HBc VLP plasmids after digestion by EcoRI and XhoI restriction enzymes analyzed by agarose gel electrophoresis. M) DNA ladder 100 bp, 1) undigested recombinant HBc VLP-E plasmid, 2) digested recombinant HBc VLP-E plasmid 3) digested recombinant HBc VLP plasmid 4) undigested recombinant HBc VLP plasmid b) SDS-PAGE analysis of the expression of HBc VLP and HBc VLP-E in E. coli. M) protein marker, 1) induced cells expressing HBc VLP-E on size 23.7 kDa, 3) induced cells expressing HBc VLP on size 20.3 kDa. 3) non-induced cell c) SDS-PAGE analysis of the purification of HBc VLP and HBc VLP-E. M1 and M2) protein marker, 1) purified HBc VLP protein, 2) purified HBc VLP-E protein d) Western Blot analysis of HBc VLP and HBc VLP-E proteins. M) protein marker, 1) purified HBc VLP-E protein, 2) purified HBc VLP protein