Table 2 List of plasmids used in this study
Plasmid | Relevant characteristics | Type of plasmid | References |
|---|---|---|---|
pEEC | Self-replicating plasmid, derived from pPMQAK plasmid (RSF1010 based); Chloramphenicol resistance | Self-replicating | [18] |
pEERM3 | Integrative plasmid, targets slr0168 neutral site of Synechocystis genome; Kanamycin resistance | Integrative | [23] |
p6EgIspS | Self-replicating plasmid, carrying the codon-optimized versions of dxs and idi genes from Coleus forskohlii and Synechocystis sp. PCC 6803, respectively, in an operon controlled by the constitutive Ptrc promoter and a bicistronic device; chloramphenicol resistance | Self-replicating, derived from pEEC | [18] |
pAgBispA | plasmid carrying codon-optimized gene encoding Abies grandis bisabolene synthase, under control of the Ptrc promoter | Self-replicating, derived from pEEC | [2] |
pEEC_PnrsB-hfq | plasmid carrying hfq gene from E. coli under control of the Nickel-inducible PnrsB promoter and RBS* | Self-replicating, derived from pEEC | This study |
pEERM3_2MEP | Integrative plasmid (targets slr0168 neutral site of Synechocystis genome) carrying the codon-optimized versions of dxs and idi genes from Coleus forskohlii and Synechocystis sp. PCC 6803, respectively, in an operon controlled by the constitutive Ptrc promoter and a bicistronic device. Kanamycin resistance | Integrative, derived from pEERM3 | This study |
pEERM3_2MEP-AgB | Integrative plasmid (targets slr0168 neutral site of Synechocystis genome) the 2 MEP genes as an operon, as well as the bisabolene synthase gene under control of the constitutive Ptrc promoter and a bicistronic device. Kanamycin resistance | Integrative, derived from pEERM3_2MEP | This study |
pEEC_Hfq_ X | plasmid carrying both the hfq cassette, controlled by PnrsB promoter, and the antisense-RNA cassette, controlled by the constitutive Ptrc1O promoter; X can be ccmA, crtB, chlP, citS, pdhB, pepC, ddH, gpi, sqs, thiG, ilvB, ilvG or atpB | Self-replicating, derived from pHfq | This study |