Mapping competitive pathways to terpenoid biosynthesis in Synechocystis sp. PCC 6803 using an antisense RNA synthetic tool

Table 1 List of genes chosen in this study as targets for knockdown

Gene name	Gene	Encoded protein	Substrate	Pathway
crtE	slr0739	Polyprenyl transferase	IPP + DMAPP	Terpenoids
chlP	sll1091	Chlorophyll synthase	GGPP	Chlorophyll a
crtB	slr1255	Phytoene synthase	GGPP	Carotenoids
sqs	sll0513	Squalene synthase	FPP	Hopanoids
thiG	slr0633	Thiamine synthase	DXP	Vitamin B₁
ddH	slr1556	d-Lactate dehydrogenase	Pyruvate	Lactate
pepc	sll0920	Phosphoenolpyruvate carboxylase	PEP	Central-carbon metabolism
atpB	sll1324	β-subunit of ATP synthase	ADP + Pi	ATP synthesis
gpi	slr1349	Glucose 6-phosphate isomerase	F6P/G6P	Sugar metabolism
ilvB ilvG	sll1981 slr2088	Acetolactate synthase large subunit Acetolactate synthase large subunit	Pyruvate	Branch-chain amino acids
ccmA	sll0934	Deoxy-d-arabinoheptulosonate 7-phosphate (DAHP) synthase	E4P and PEP	Aromatic amino acids

ISSN: 1475-2859