Table 1 The strains and plasmids used in this study
Strains/plasmids | Description | Source |
|---|---|---|
Strains | ||
 E. coli JM109 | General cloning host | Our lab |
 E. coli ET12567 (pUZ8002) | Cmr, Kmr, donor strain for conjugation | Our lab |
 E. coli BL21(DE3) | Strain for protein expression and purification | Our lab |
 S. rimosus M527 | Parental strain, rimocidin producer | CCTCC M2013270 |
 M527-ER | M527 containing integrative vector pIB139-rimR2 | This work |
 M527-KR | M527 containing integrative vector pSET152-KR | This work |
 M527-21R | M527 containing integrative vector pSET152-21R | This work |
 M527-57R | M527 containing integrative vector pSET152-57R | This work |
 M527-NR | M527 containing integrative vector pSET152::rimR2 | This work |
 M527-pSET152 | M527 containing integrative vector pSET152 | This work |
 M527-ΔrimR2 | rimR2 gene disruption mutant, derived from M527 strain | This work |
 M527-ΔrimR2/ pSET152 | Mutant M527-ΔrimR2 with integrative plasmid pSET152 | This work |
 M527-ΔrimR2/ pSET152::rimR2 | rimR2 complemented strain, mutant M527-ΔrimR2 with integrative plasmid pSET152::rimR2 | This work |
Plasmids | ||
 pIB139 | Apr, permE*, integrative vector, based on pSET152 | Our lab |
 pSET152 | Apr, no promoter, integrative vector | Our lab |
 pWHU2653 | Scas9, sgRNA cloning cassette, codA(sm), aprr, ori(coEl) | Zeng et al. [49] |
 pGUS-SPL21 | gusA under the control of promoter SPL21 | Siegl et al. [50] |
 pGUS-SPL57 | gusA under the control of promoter SPL57 | Siegl et al. [50] |
 pGUS-ermE* | gusA under the control of synthetic promoter permE* | Prof. Luzhetskyy |
 pDR4 | Hygr, kasOp*, based on pSET152 | Wang et al. [51] |
 pSPL21-rimR2 | Derived from pGUS-SPL21, gusA was replaced by rimR2 gene | This work |
 pSPL57-rimR2 | Derived from pGUS-SPL57, gusA was replaced by rimR2 gene | This work |
 permE*-rimR2 | Derived frompGUS-ermE*, gusA was replaced by rimR2 gene | This work |
 pDR4-rimR2 | Derived from pDR4, rimR2 gene under the control of kasOp* | This work |
 pSET152::rimR2 | Derived frompSET152, harboring rimR2 gene driven by its own promoter | This work |
 pET32a | Ampr, Expression vector | Our lab |
 pET32a-rimR2 | Derived from pET32a, harboring rimR2 gene driven by T7 promoter | This work |