Fig. 4

Screening of genes related with KA biosynthesis using kojic acid producing A. niger as a platform. a Colony Phenotype of 10 mutant strains grown on PDA for 4 days. The strains are listed as follows: the marker-less kojA overexpression strain S2132 (OEkojA) used as the parent strain, ASPNIDRAFT_50239 deletion mutant S2624 (Δ50239), ASPNIDRAFT_171597 deletion mutant S2922 (Δ171597), RNAi strain targeting ASPNIDRAFT_42619 S2930 (RNAi-42619), ASPNIDRAFT_189096 deletion mutant S2924 (Δ189096), ASPNIDRAFT_43217 deletion mutant S2929 (Δ43217), ASPNIDRAFT_53284 deletion mutant S2626 (Δ53284), RNAi strain targeting ASPNIDRAFT_56871 S2933 (RNAi-56871), ASPNIDRAFT_209619 deletion mutant S2430 (Δ209619), ASPNIDRAFT_186610 deletion mutant S2435 (Δ186610), ASPNIDRAFT_131173 deletion mutant S2437 (Δ131173). b KA production by the parent strain S2132 and the 10 derivative mutant strains. The strains are listed as described above. The titers of KA produced by A. niger S2132 and 10 derivative mutants in shake flask cultivations for 5 d and 7 d were shown